Diversity of leaf morphometric parameters in natural Greek populations of Arbutus unedo

Authors

  • Despoina Eleni Politi Laboratory of Forest Genetics, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki Author
  • Filippos A. Phil Aravanopoulos Laboratory of Forest Genetics, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki Author

DOI:

https://doi.org/10.2478/foecol-2022-0013

Keywords:

contrasting sites, morphology, natural variation, strawberry tree

Abstract

This paper investigates leaf morphology variation of the strawberry tree (Arbutus unedo) within and between two natural contrasting populations of significant latitudinal difference (Kassandreia, Chalkidiki and Ancient Olympia, Peloponnese). This study employed 11 leaf size and shape parameters, recorded by image processing and analyzing software. The results showed that in the measurements of central tendency (parameter means) the northern population of Kassandreia presented the highest values, while in contrast the highest values in the measurements of spread were found in the southern population of Ancient Olympia. Moreover, statistically significant differences between populations were detected in leaf size, but not in leaf shape parameters. Results are discussed in the context of their value in studying quantitative population differentiation and laying the basis of more advanced studies.

References

Abrumoff, M.D., Magalhaes, P.J., Ram, S.J., 2004. Image processing with ImageJ. Biophotonics International, 11: 36–42.

Akbarian, M.R., Tabari, M., Akbarinia, M., Zarafshar, M., Meave, J.A., Yousefzadeh, H., Sattarian,A., 2011. Effects of elevational gradient on leaf and stomatal morphology of Caucasian alder (Alnus sabcurdata) in the Hyrcanian forest, Iran. Folia Oecologica, 38: 1–7.

Aravanopoulos, F.A., 2005. Phenotypic variation and population relationships of chestnut (Castanea sativa) in Greece, revealed by multivariate analysis of leaf morphometrics. Acta Horticulturae, 693: 223–240. https://doi.org/10.17660/ActaHortic.2005.693.28

Aravanopoulos, F.A., 2010. Contribution of leaf morphometrics in the study of genetic entries in Salix. Electronic Journal of Plant Breeding, 1: 1320–1328.

Benavides, R., Carvalho, B., Bastias, C.C., López-Quiroga, D., Mas, A., Cavers, S., Gray, A., Albet, A., Alía, R., Ambrosio, O., Aravanopoulos, F., Auñón, F., Avanzi, C., Avramidou, E.V., Bagnoli, F., Ballesteros, E., Barbas, E., Bastien, C., Bernier, F., Bignalet, H., Bouic, D., Brunetto, W., Buchovska, J., Cabanillas-Saldaña, A.M., Cheval, N., Climent, J.M., Correard, M., Cremer, E., Danusevičius, D., Dauphin, B., Del Caño, F., Denou, J.-L., Dokhelar, B., Dourthe, R., Farsakoglou, A.-M., Fera, A., Fonti, P., Ganopoulos, I., García del Barrio, J.M., Gilg, O., González-Martínez, S.C., Graf, R., Grivet, D., Gugerli, F., Hartleitner, Ch., Heer, K., Hollenbach, E., Hurel, A., Issehuth, B., Jean, F., Jorge, V., Jouineau, A., Kappner, J.-P., Kärkkäinen, K., Kesälahti, R., Knutzen, F., Kujala, S.T., Kumpula, T., Labriola, M., Lalanne, C., Lambertz, J., Lascoux, M., Le Provost, G., Liesebach, M., Malliarou, E., Marchon, J., Mariotte, N., Martínez-Sancho, E., Matesanz, S., Meischner, H., Michotey, C., Milesi, P., Morganti, S., Myking, T., Nilsen, A.E., Notivol, E., Opgenoorth, L., Østreng, G., Pakull, B., Piotti, A., Plomion, Ch., Poinot, N., Pringarbe, M., Puzos, L., Pyhäjärvi, T., Raffin, A., Ramírez-Valiente, J.A., Rellstab, Ch., Richter, S., Robledo-Arnuncio, J.J., San Segundo, S., Savolainen, O., Schneck, V., Schueler, S., Scotti, I., Semerikov, V., Sønstebø, J.H., Spanu, I., Thevenet, J., Tollefsrud, M.M., Turion, N., Vendramin, G.G., Villar, M., Westin, J., Fady, B., Valladares, F., 2021. The GenTree Leaf Collection: inter- and intraspecific leaf variation in seven forest tree species in Europe. Global Ecology and Biogeography, 30: 590–597. https://doi.org/10.1111/geb.13239

Blazakis, K.N., Kosma, M., Kostelenos, G., Baldoni, L., Bufacchi, M., Kalaitzis, P., 2017. Description of olive morphological parameters by using open access software. Plant Methods, 13: 111. https://doi.org/10.1186/s13007-017-0261-8

Caudullo, G., Welk, E., San-Miguel-Ayanz, J., 2017. Chorological maps for the main European woody species. Data in Brief, 12: 662–666. https://doi.org/10.1016/j.dib.2017.05.007

Celikel, G., Demirsoy, L., Demirsoy, H., 2008. The strawberry tree (Arbutus unedo L.) selection in Turkey. Scientia Horticulturae, 118: 115–119. https://doi.org/10.1016/j.scienta.2008.05.028

Christou, Α.Κ., Aravanopoulos, F.A., 1997. Genetic diversity of Pinus brutia Ten. in Cyprus based on a multivariate analysis of morphological and anatomical traits. Scientific Annals, School of Forestry and Natural Environment, Aristotle University of Thessaloniki, 40: 929–949.

Dickinson, T.A., 1986. Topodeme differentiation in Ontario taxa of Crataegus (Rosaceae: Maloideae): leaf morphometric evidence. Canadian Journal of Botany, 64: 2738–2747. https://doi.org/10.1139/b86-363

Fady, B., Aravanopoulos, F.A., Alizoti, P., Matyas, C., von Wuhlisch, G., Westergren, M., Belletti, P., Cvjetkovic, B., Ducci, F., Huber, G., Kelleher, C.T., Khaldi, A., Bou Dagher Kharrat, M., Kraigher, H., Kramer, K., Mühlethaler, U., Peric, S., Perry, A., Rousi, M., Sbay, H., Stojnic, S., Tijardovic, M., Tsvetkov, I., Varela, M.C., Vendramin, G.G., Zlatanov, T., 2016. Evolution-based approach needed for the conservation and silviculture of peripheral forest tree populations. Forest Ecology and Management, 375: 66–75. https://doi.org/10.1016/j.foreco.2016.05.015

Ferreira, T., Rasband W., 2012. ImageJ User Guide. ImageJ/ Fiji 1.46. [cit. 2022-02-25]. https://imagej.nih.gov/ij/download.html

Hamrick, J.L., 1983. The distribution of genetic variation within and among natural plant populations. In Shonewald-Cox, C., Chambers, S., MacBryde, B., Thomas, W. (eds). Genetics and conservation. Menlo Park, California: Benjamin/Cummings, p. 335–348.

Hatziskakis, S., Tsiripidis, I., Papageogiou, A.C., 2011. Leaf morphological variation in beech (Fagus sylvatica L.) populations in Greece and its relation to their post-glacial origin. Botanical Journal of the Linnean Society, 165: 422–436. https://doi.org/10.1111/j.1095-8339.2011.01124.x

HNMS (Hellenic National Meteorological Service), 2022. Climatic atlas of Greece. [cit. 2022-06-06]. http://climatlas.hnms.gr/sdi/

IBM, 2020. IBM SPSS statistics for Windows, version 27.0. Armonk. NY: IBM Corp.

Kelleher, C.T., 2013. In search of the origins of Ireland’s arctic and Mediterranean Plants. In Jebb, M., Crowley, C. (eds). Secrets of the Irish landscape. Cork, Ireland: Cork University Press, p. 39–45.

Kleinschmit, J.R.G., Bacilieri, R., Kremer, A., Roloff, A., 1995. Comparison of morphological and genetic traits of pedunculate oak (Q. robur L.) and sessile oak (Q. petraea (Matt.) Liebl.). Silvae Genetica, 44: 256–269.

Korakis, G., 2015. Forest botany. Athens: Association of Hellenic Academic Libraries Publ. 620 p.

Kourmpetis, Y.A.I., Aravanopoulos, F.A., 2010. A comparative fluctuating asymmetry study between two walnut (Juglans regia L.) populations may contribute as an early signal for bio-monitoring. iForest, 3: 150–152. https://doi.org/10.3832/ifor0552-003

Leimu, R., Fischer, M., 2008. A meta-analysis of local adaptation in plants. PLoS ONE, 3: e4010. https://doi.org/10.1371/journal.pone.0004010

Li, Y., Zou, D., Shrestha, N., Xu, X., Wang, Q., Jia, W., Wang, Z., 2020. Spatiotemporal variation in leaf size and shape in response to climate. Journal of Plant Ecology, 13: 87–96. https://doi.org/10.1093/jpe/rtz053

Lopes, L., Sa, O., Pereira, J.A., Baptista, P., 2012. Genetic diversity of Portuguese Arbutus unedo L. populations using leaf traits and molecular markers: an approach for conservation purposes. Scientia Horticulturae, 142: 57–67. https://doi.org/10.1016/j.scienta.2012.04.031

Meijering, E., Dzyubachyk, O., Smal, I., van Cappellen, W.A., 2009. Tracking in cell and developmental biology. Seminars in Cell and Developmental Biology, 20: 894–902. https://doi.org/10.1016/j.semcdb.2009.07.004

Mitton, J.B., 1978. Relationship between heterozygosity for enzyme loci and variation for morphological characters in natural populations. Nature, 273: 661–662.566385 https://doi.org/10.1038/273661a0

Mohebi Bijarpasi, M.M., Shahraji, T.R., Lahiji, H.S., 2019. Genetic variability and heritability of some morphological and physiological traits in Fagus orientalis Lipsky along an elevation gradient in Hyrcanian forests. Folia Oecologica, 46: 45–53. https://doi.org/10.2478/foecol-2019-0007

Neophytou, Ch., Palli, G., Dounavi, A., Aravanopoulos, F.A., 2007. Morphological differentiation and hybridization between Quercus alnifolia and Quercus coccifera L. (Fagaceae) in Cyprus. Silvae Genetica, 56: 271–277. https://doi.org/10.1515/sg-2007-0038

Nicotra, A.B., Leigh, A., Boyce, C.K., Jones, C.S., Niklas, K.J., Royer, D.L., Tsukaya, H., 2011. The evolution and functional significance of leaf shape in the angiosperms. Functional Plant Biology, 38: 535–552. https://doi.org/10.1071/FP11057

Oliveira, I., Baptista, P., Bento, A., Pereira, J.A., 2011. Arbutus unedo L. and its benefits on human health. Journal of Food and Nutrition Research, 50: 73–85.

Peppe, D.J., Royer, D.L., Cariglino, B., Oliver, S.Y., Newman, S., Leight, E., Enikolopov, G., FernandezBurgos, M., Herrera, F., Adams, J.M., Correa, E., Currano, E.D., Erickson, J.M., Hinojosa, L.F., Hoganson, J.W., Iglesias, A., Jaramillo, C.A., Johnson, K.R., Jordan, G.J., Kraft, N.J.B., Lovelock, E.C., Lusk, C.H., Niinemets, U., Peñuelas, J., Rapson, G., Wing, S.L., Wright, I.J., 2011. Sensitivity of leaf size and shape to climate: global patterns and paleoclimatic applications. New Phytologist, 190: 724–39. https://doi.org/10.1111/j.1469-8137.2010.03615.x

Rajora, O.P., Zsuffa, L., Dancik, B.P., 1991. Allozyme and leaf morphological variation of eastern cottonwood at the northern limits of its range in Ontario. Forest Science, 37: 668–702 https://doi.org/10.1093/forestscience/37.2.688/

Stojnic, S., Orlovic, S., Miljkovic, D., Wuehlisch, G., 2016. Intra and inter provenance variations in leaf morphometric traits in European beech (Fagus sylvatica L.). Archives of Biological Sciences, 68: 781–788. DOI: https://doi.org/10.2298/ABS151008064S

Sulusoglu, M., Cavusoglu, A., Erkal, S., 2011. Arbutus unedo L. (Strawberry tree) selection in Turkey Samanli mountain locations. Journal of Medicinal Plants Research, 5: 3545–3551.

Williams J., William, B. Howard, D., 2001. Reproductive processes in two oak (Quercus) contact zones with different levels of hybridization. Heredity, 87: 680–690.11903563 https://doi.org/10.1046/j.1365-2540.2001.00968.x

Wright, I.J., Dong, N., Maire, V., Prentice, I.C., Westoby, M., Diaz, S., Gallagher, R.W., Jacobs, B.F., Kooyman, R., Law, E.A., Leishman, M.R., Niinemets, Ü., Reich, P.B., Sack, L., Villar, R., Wang, H., Wilf, P., 2017. Global climatic drivers of leaf size. Science, 357: 917–921.28860384 https://doi.org/10.1126/science.aal4760

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2022-08-05

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