Stomatal variations and their position relative to leaf epidermal cells in ten Maple species
DOI:
https://doi.org/10.2478/foecol-2024-0009Keywords:
Acer, form and position, principal component analysis, stomataAbstract
In the present study, we investigated the structure of stomata in seven native species of Hyrcanian forests (Acer hyrcanum, A. velutinum Boiss., A. campestre, A. platanoides L., A. cappadocicum, A. monspessulanum, A. amazandaranicum), as well as non-native species that have fully adapted (A. negundo, A. negundo variegatum, and A. palmatum). We used light and electron microscopy to determine the form and position of the stomata in relation to the leaf epidermal cells. The length, width, shape, area, perimeter, and stomatal density were all measured. Our findings revealed that the stomata type of A. negundo varengiayum, A. campestre, A. hyrcanum, A. mazandaranicum and A. monsspesulanum is anomocytic, A. platanoides and A. cappadocicum have anomocytic stomata with wavy subsidiary cells, while A. palmatum has anisocytic stomata and A. velutinum has parasitic stomata. A. negundo has actinocytic stomata. Regarding the location of stomata relative to adjacent epidermal cells, we identified three types. In the first type, the stomata were flush with adjacent epidermal cells (A. cappadocicum, A. negundo, A. platanoides). In the second type, the stomata were higher (A. negundo variegatum), and in the third type, the stomata were lower (A. velutinum, A. monspesulanom, A. campestre, A. mazandaranicum, A. hyrcanum). The principal component analysis was used to determine the essential stomatal traits in differentiating between species. We also investigated the distribution of trees in the coordinate axis space based on two main components and performed cluster analysis based on stomatal characteristics. A. platanoides, A. negundo, A. negundo variegatum were in one cluster, while the other species were in separate clusters. The calculation of dissimilarity among the studied species revealed the lowest similarity between A. negundo and A. hyrcanum and the highest similarity between A. campestre and A. mazandaranicum. The results of the discriminant analysis identified stomatal density as the essential factor in differentiation between the studied species.
References
Alushi, I., Veiz, X., 2020. Effects of air pollution on stomatal responses, including paleoatmospheric CO2 concentration, in leaves of Hedera helix. Albanian Journal of Agricultural Sciences, 19 (1): 21–28.
Amran, A.N., Noor, N.N.N., Mohamed, F., 2023. Micro-morphological analyses of stomata and epidermal cellamong different species in the genus Premna L. (Lamiaceae). Malaysian Journal of Microscopy, 19 (1): 319–332.
Ao, C., Ye, C., Zhang, H., 2007. A systematic investigation of leaf epidermis in Camellia using light microscopy. Biologia, 62: 157–162. https://doi.org/10.2478/s11756-007-0024-5
Asadi, F., Sharifnia, F., Salimpour, F. Majd, A., 2019. Using micro-morphological fruit characters in resolving some of ambiguities in Iranian Acer L. (Sapindaceae) species. Biodiversitas, 20 (1): 297–304. https://doi.org/10.13057/biodiv/d200134
Ba, Q., Khan, W., Pang, J., Gong, T., Quan, B., Duo, R., Shu, H., 2023. Comparative analysis of foliar epidermal anatomical traits and their taxonomic relevance in Lilium pumilum, L. brownii, and L.dawidi. Microscopy Research and Technique. https://doi.org/10.1002/jemt.24443
Baranova, M., 1992. Principles of comparative stomato-graphic studies of flowering plants. The Botanical Review, 58 (1): 49–99. https://doi.org/10.1007/BF02858543
Belhadj, S., Derridj, A., Moriana, A., Gijon, M.D.C., Mevy, J.P. Gauquelin, T., 2011. Comparative analysis of stomatal characters in eight wild atlas pistachio populations (Pistacia atlantica Desf. Anacardiaceae). International Research Journal of Plant Science, 2: 60–69.
Chapolagh Paridari, I., Jalali, Gh., Sonboli, A. Zarafshar, M., 2012. Leaf, stomata and trichomemorphology of the species in Carpinus genus. Taxonomy and Biosystematics, 10: 12–26.
Cobanglu, H., Kulac, S., Şevik, H., 2023. Effect of drought and UV-B stress on stoma characteristics in two maple species. Kastamonu University Journal of Engineering and Sciences, 9 (1): 1–9. https://doi.org/10.55385/kastamonujes.1285522
Gang, Z., Liu, B., Rohwer, J.G., Ferguson, D.K., Yang, Y., 2021. Leaf epidermal micromorphology defining the clades in Cinnamomum (Lauraceae). PhytoKeys, 182: 125–148. https://doi.org/10.3897/phytokeys.182.67289
Ghahreman, A., 1997. Iran chromophytes (systematic plant). Vol. 4. Tehran: Tehran University Publication Center. 752 p.
Gray, A., Liu, L, Facette, M., 2020. Flanking support: how subsidiary cells contribute to stomatal form and function, Frontiers in Plant Science, 11: 881. DOI: 10.3389/fpls. 2020.00881. https://doi.org/10.3389/fpls.
Grimm, G.W., Denk, T. Hemleben, V., 2007. Evolutionary history and systematics of Acer section Acer – a case study of low-level phylogenetics. Plant Systematics and Evolution, 267: 215–253. https://doi.org/10.1007/s00606-007-0572-8
Grimm, G.W., Denk, T., 2014. The Colchic region as refuge for relict tree lineages: cryptic speciation in field maples. Turkish Journal of Botany, 38 (6): 1050–1066. https://doi.org/10.3906/bot-1403-87
Guo, J.S., Bush, S.E., Hultine, K.R., 2022. Temporal variation in stomatal sensitivity to vapour pressure deficit in western riparian forests. Functional Ecology, 36 (7): 1599–1611. https://doi.org/10.1111/1365-2435.14066
Haron, N.W, Moore, D.M., 1996. The taxonomic significance of leaf micro morphology in the genus Eugenia L. (Myrtaceae). Botanical Journal of the Linnean Society, 120 (3): 265–277. https://doi.org/10.1111/j.1095-8339.1996.tb00776.x
Harrison, E.L., Arce Cubas, L., Gray, J.E., Hepworth, C., 2020. The influence of stomatal morphology and distribution on photosynthetic gas exchange. The Plant Journal, 101 (4): 768–779. https://doi.org/10.1111/tpj.14560
Hatziskakis, S., Tsiripidis, I., Papageogioui, A.C., 2011. Leaf morphological variation in beech (Fagus sylvatica L.) populations in Greece and its relation to their post-glacial origin. Botanical Journal of the Linnean Society, 165 (4): 422–436. https://doi.org/10.1111/j.1095-8339.2011.01124.x
Holmes, M.G., Keiller, D.R., 2002. Effects of pubescence and waxes on the reflectance of leaves in the ultraviolet and photosynthetic wavebands: a comparison of a range of species. Plant, Cell and Environment, 25: 85–93. http://dx.doi.org/10.1046/j.1365-3040.2002.00779.x.
Hong, T., Lin, H., He, D., 2018. Characteristics and correlations of leaf stomata in different Aleurites montana provenances. PLOS ONE 13 (12): e0208899. https://doi.org/10.1371/journal.pone.0208899
Karimi, Z., 2021. Leaf anatomical and morphological characters of some species of Acer. Journal of Plant Research (Iranian Journal of Biology), 34 (3): 616–631.
Kordalivand, A., Payamnoor, V., Sattarian, A., Mohammadi, J., 2015. Different types of leaf stomata in genus Betula L. in Iran. Journal of Wood and Forest Science and Technology, 22 (2): 55–73.
Liu, J., Wang, l., Xing, Y., 2014. Morphology structure of leaf epidermis of Maloideae in Heilongjiang. Journal of Chinese Electron Microscopy Society, 3 (1): 69–76.
Luo, Y., Zhou, ZK., 2001. Cuticle of Quercus Sugen. Cyclobalanopsis (Oerst.) chneid. (Fagaceae). Acta Phytophysiologica Sinica, 39: 489–501.
McElwain, J.C., 2004. Climate-indipendent paleoaltimetry using stomatal density in fossil leaves as proxy for CO2 partial pressure. Geology, 32: 1017–1020.
Miles, L.M., Jeanne, A.M., Robert, D.W. 1995. Provenance and progeny variation in growth and frost tolerance of Casuarina cunninghmiana in California, USA. Forest Ecology and Management, 79: 161–171. https://doi.org/10.1016/0378-1127(95)03612-1
Mohtashamian, M., Attar, F., Kavousi, K., Masoudi-Nejad, A., 2017. Biogeography, distribution and conservation status of maples (Acer L.) in Iran. Trees, 31: 1583–1598. https://doi.org/10.1007/s00468-017-1571-1
Oyeleke, M.O., Abdul Rahaman, A.A., Oladele, F.A., 2004. Stomatal anatomy and transpiration rate in some afforestation tree species. Journal of the Nigerian Society for Experimental Biology, 4 (2): 83–90.
Pourkhabbaz, A., Rastin, N., Olbrich, A., Langenfeld-Heyser, R., Andrea, P.A., 2010. Influence of environmental pollution on leaf properties of urban plane trees, Platanus orientalis L. Bulletin of Environmental Contamination Toxicology, 85: 251–255. https://doi.org/10.1007/s00128-010-0047-4.
Prabhakar, M., 2004. Structure, delimitation, nomenclature and classification of stomata. Journal of Integrative Plant Biology, 46 (2): 242.
Salehi, M., Fotouhi Ghazvini, R., 2016. Effect of acid rain on morphological and physiological characteristics of Persian Maple (Acer velutinum Boiss). Journal of Plant Process and Function, 5 (15): 23–32.
Schoettle, A.W., Rochelle, S.G., 2000. Morphological variation of Pinus flexilis (Pinaceae), a bird-dispersed pine, across a range of elevations. American Journal of Botany, 87: 1797–1806.
Uzunova, K.R., 1999. A comparative study of leaf epidermis in European Corylaceae. Feddes Repertorium,110: 209–218. https://doi.org/10.1002/fedr.19991100307
Van Cotthem, W.R.J., 1970. A classification of stomata types. Botanical Journal of the Linnean Society, 63 (3): 235–246. https://doi.org/10.1111/j.1095-8339.1970.tb02321.x
Wang, Z.X., Zhang, H., Chen, L., Zhao, Y.M., Zhai, J.W., Chen, S.P., Wu, S.S., 2020. Leaf epidermal micro-morphology and taxonomic significance of 15 species of Pleione. Acta Botanica Boreali-Occidentalia Sinica, 40: 1527–1538.
Yosefzadeh, H., Hosseinzadeh Colagar, A., Tabari, M., Sattarian, A., Assadi, M., 2010. Recognition of different stomata types of Tilia spp. in hyrcanian forests. Taxonomy and Biosystematics, 5: 17–28.
Zhu, L.L., Wu, J.C., Peng, X.M., Zheng, Y.M., Zhang, Y.P., 2016. Phenotypic difference among species and a variation type of Azadirachta. Forest Research, 29 (2):162–166.
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