Salicylic acid-mediated salt tolerance in date palms: comparative analysis of application methods
Keywords:
antioxidant enzymes, foliar applications, ion homeostasis, Phoenix dactylifera L., salicylic acid, salinity stress tolerance, trunk injectionAbstract
In arid environments, tree growth and ecosystem function are limited by salinity stress. Herein, we describe the role of salicylic acid (SA) in ameliorating salt tolerance in date palms (Phoenix dactylifera L., cv. Sayer), a woody species characteristic of arid, salinized environments. We examined the role of salicylic acid (SA) delivered via trunk injection and foliar treatment in the tree’s physiological acclimatization strategy. Trunk injection of SA at 200 ppm resulted in the highest plant height. SA treatments were associated with increased antioxidant activities (peroxidase and superoxide dismutase), decreased malondialdehyde, and fine-tuned hormone homeostasis (increased auxin and decreased abscisic acid). The trunk injection has much higher efficacy, providing important insights into the adaptation mechanisms of the tree and the efficacy of bioactive compound delivery from the injection site. Our results have implications for tree survival in increasingly salinized arid systems, and together, these findings represent progress toward a mechanistic understanding of ecophysiological pathways of adaptation to environmental stress in trees.
References
Angefini, R., Manes, F., Federico, R., 1990. Spatial and
functional correlation between diamine-oxidase and
peroxidase activities and their dependence upon de-etio-
lation and wounding in chick-pea stems R. Planta, 182:
89–96.
Alharbi, K., Al-osaimi, A.A., Alghamdi, B.A., 2022. Sodium
chloride (NaCl)-induced physiological alteration and
oxidative stress generation in Pisum sativum (L.): a toxi-
city assessment. ACS Omega, 7 (24): 20819–20832.
https://doi.org/10.1021/acsomega.2c01427
Al-Mayahi, A.M.W., Kalaf, Y.N., Abdul-Sahib, A.M., Abdul
-Sahib, I.M., Al-Sharifi, A.A., 2024. Anatomical study
of adventitious bud regeneration from shoot tip of date
palm (Phoenix dactylifera L.) c.v Barhee in vitro. Basrah
Journal of Date Palm Research, 23 (1): 116–126
Archer, L., Albrecht, U., 2023. Evaluation of trunk injection
techniques for systemic delivery of huanglongbing
therapies in Citrus. Horticultural Science, 58 (7): 768–
778. https://doi.org/10.21273/HORTSCI17172-23
Archer, L., Crane, J.H., Albrecht, U., 2024. Trunk injection
as a tool to deliver plant protection materials an
overview of basic principles and practical considera-
tions. Horticulturae, 8: 552. https://doi.org/10.3390/
horticulturae10040351
Assaha, D.V.M., Ueda, A., Saneoka, H., Al-Yahyai, R.,
Yaish, M.W., 2017. The role of Na (+) and K (+) trans-
porters in salt stress adaptation in glycophytes. Frontiers
in Physiology, 8: 509. https://doi.org/10.3389/fphys.2017.
00509
Barwal, S.K., Shah, S.H., Pawar, A., Siddiqui, M.H., Ag-
nihotri, R.K., Vimala, Y. , Wani, S.H., 2024. Mecha-
nistic insights of salicylic acid-mediated salt stress tole-
rance in Zea mays L. seedlings. Heliyon, 10 (14): e34486.
https://doi.org/10.1016/j.heliyon.2024.e34486
Bates, L., Waldren, S., Teare , R.P., Rapid, I.D., 1973. Rapid
determination of free proline for water stress studies.
Plant and Soil, 39: 205–207. https://doi.org/10.1007/
BF00018060
Beauchamp, C., Fridovich, I., 1971. Superoxide dismutase:
improved assays and an assay applicable to acrylamide
gels. Analytical Biochemistry, 44: 276–287.
Cresser, M.S., Parsons, J.W., 1979. Sulphuric – perchloric
acid digestion of plant material for the determination of
nitrogen, phosphorus, potassium, calcium, and magne-
sium. Analytical Chimica Acta, 109: 431–436. https://
doi.org/10.1016/S0003-2670(01)84273-2
Easlon, H.M.,Bloom, A.J., 2014. Easy Leaf Area: automated
digital image analysis for rapid and accurate measure-
ment of leaf area. Applications in Plant Sciences, 2 (7):
1400033. https://doi.org/10.3732/apps.1400033
Filgueiras, C.C., Martins, A.D., Pereira, R.V, Willett, D.S.,
2019. The ecology of salicylic acid signaling: primary,
secondary and tertiary effects with applications in agri-
culture. International Journal of Molecular Sciences,
23: 5851. https://doi.org/10.3390/ijms20235851
Fu, H.,Yang, Y., 2023. How plants tolerate salt stress. Current
Issues in Molecular Biology, 45: 5914–5934. https://doi.
org/10.3390/cimb45070374
Hameed, A., Ahmed, M.Z., Hussain, T., Aziz, I., Ahmad, N.,
Gul, B., Nielsen, B.L., 2021. Effects of salinity stress
on chloroplast structure and function. Cells, 10 (8):
34440792. https://doi.org/10.3390/cells10082023
Han, X., Zhao, Y., Chen, Y., Xu, J., Jiang, C., Wang, X., Zhuo,
R., Lu, M.-Z., Zhang, J., 2022. Lignin biosynthesis and
accumulation in response to abiotic stresses in woody-
plants. Forestry Research, 2: 9. https://doi.org/10.48130/
FR-2022-0009
Hasanuzzaman, M., Raihan, M.R.H., Masud, A.A.C., Rah-
man, K., Nowroz, F., Rahman, M., Nahar, K., Fuji-
ta, M., 2021. Regulation of reactive oxygen species
and antioxidant defense in plants undersalinity. Inter-
national Journal of Molecular Sciences, 22 (17). https://
doi.org/10.3390/ijms22179326
Heath, R.L., Packer, L., 1968. Photoperoxidation in iso-
lated chloroplasts I. Kinetics and stoichiometry of fatty
acid peroxidation. Archives of Biochemistry and Bio-
physics, 125: 189–198.
Hussain, M.S., Naeem, M.S., Tanvir, M.A., Nawaz, M.F.,
Abd-Elrahman, A., 2021. Ecophysiological evalua-
tion of multipurpose tree species to ameliorate saline
soils. International Journal of Phytoremediation, 23 (9):
969–981. https://doi.org/10.1080/15226514.2020.1871321
299
Islam, S.M.N., Paul, N., Rahman, M.M., Haque, M.A.,
Rohman, M.M., Mostofa, M.G., 2023. Salicylic acid
application mitigates oxidative damage and improves
the growth performance of barley under drought stress.
Phyton-International Journal of Experimental Botany,
92 (5): 1513–1537. https://doi.org/10.32604/phyton.2023.
025175
Kashif, M.H., Feng, J., Dai, R., Fan, Y., Xia, Y., Liu, Z.,
2024. Salicylic acid-mediated alleviation of salt stress:
Insights from physiological and transcriptomic analysis
in Asarum sieboldii Miq. Chemosphere, 362: 142604.
https://doi.org/10.1016/j.chemosphere.2024.142604
Kopecká, R., Černý, M., 2024. Xylem sap proteome analysis
provides insight into root-shoot communication inre
sponse to flg22. Plants, 13 (14): 1983. https://doi.org/
10.3390/plants13141983
Kumar, r., Sagar, V., Verma, V.C., Kumari, M., Gujjar,
R.S., Goswami, S.K., Kumar, Jha S., Pandey, H., Dubey,
A.K., Srivastava, S., Singh, S.P., Mall, A.K., Pathak,
A.D., Singh, H., Jha, P.K., Prasad, P.V.V., 2023.
Drought and salinity stresses induced physio-biochemical
changes in sugarcane: an overview of tolerance mecha-
nism and mitigating approaches. Frontiers in Plant Scie-
nce, 14: 1225234. https: //doi.org/10.3389/fpls.2023.1225234
Li, J., Yang, Y., 2023. How do plants maintain pH and ion ho-
meostasis under saline-alkali stress ? Frontiers in Plant
Science, 14: 1217193. https://doi.org/10.3389/fpls.2023.
1217193
Li, X., Liu, J., Yang, G., Chen, Y., Jiang, L., He, X., Lv, G.,
2025. Biodiversity and ecosystem multifunctionality in
arid deserts: water effects and the contributions of bio-
tic and abiotic factors. Ecological Indicators, 178: 113988.
https://doi.org/10.1016/j.ecolind.2025.113988
Lichtenthaler, H.K., Wellburn, A.R., 1983. Determina-
tions of total carotenoids and chlorophylls a and b of
leaf extracts in different solvents. Biochemical Socie-
ty Transactions, 11 (5): 591–592. https://doi.org/10.1042/
bst0110591
Liu, C., Jiang, X., Yuan, Z., 2024. Plant responses and ada-
ptations to salt stress : a review. Horticulturae, 10: 1221.
https://doi.org/https://doi.org/10.3390/horticulturae
10111221
Liu, J., Shabala, S., Shabala, L., Zhou, M., Meinke, H.,
Venkataraman, G., Chen, Z., Zeng, f., Zhao, Q., 2019.
Tissue-specific regulation of Na+ and K+ transporters ex-
plains genotypic differences in salinity stress toleran-
ce in rice. Frontiers in Plant Science,10: 2019. https://
www.frontiersin.org/journals/plant-science/articles/
10.3389/fpls.2019.01361
Lu, X., Luo, C., Wu, J., Deng, Y., Mu, X., Zhang, T., Yang,
X., Liu, Q.; Li, Z., Tang, S., Hu, Y., Du, Q., Xu, J., Xie,
R., 2023. Ion channels and transporters regulate nutrient
absorption in health and disease. Journal of Cellular
and Molecular Medicine, 27 (18): 2631–2642. https://
doi.org/10.1111/jcmm.17853
Musazade, E., Mrisho, I.I., Feng, X., 2025. Auxin metabo-
lism and signaling: integrating independent mechanisms
and crosstalk in plant abiotic stress responses. Plant
Stress, 18: 101034. https://doi.org/10.1016/j.stress.2025.
101034
Müller, J., Scheuring, D., 2025. Salicylic acid: new pathways
arising?. Frontiers in Plant Science, 16: 1681791. https://
doi.org/10.3389/fpls.2025.1681791
Osei, R., Yang, C., Cai, F., Boamah, S., 2022. Mechanisms
underlying salicylic acid on root parameters and antioxi-
dant enzyme activity of potato plant under Lelliottia
amnigena stress. Chilean Journal of Agricultural Research,
82 (2): 225–233. https://doi.org/10.4067/S0718-5839202
2000200225
Ouyang, N., Hu, W., Meng, J., Wang, B., 2025. Amino acid
derivatives as regulatory molecules : mechanisms in plant
growth and stress tolerance. The Crop Journal, 13: 668–
680. https://doi.org/10.1016/j.cj.2025.04.005
Panozzo, A., Bolla, P.K., Barion, G., Botton, A., 2025.
Phytohormonal regulation of abiotic stress tolerance,
leaf senescence and yield response in field crops: a com-
prehensive review. BioTech, 14: 14. https://doi.org/
10.3390/biotech14010014
Rastegar, S., Rahemi, M., Zargari, H., 2011. Changes in
endogenous hormones in fruit during growth and develop-
ment of date palm fruits. American-Eurasian Journal of
Agricultural and Environmental Sciences,11 (2): 140–148.
Rao, M.J., Duan, M., Zhou, C., Jiao, J., Cheng, P., Yang,
L., Rauf, A., Ai, J., Zheng, B., 2025. Antioxidant de-
fense system in plants: reactive oxygen species produc-
tion, signaling, and scavenging during abiotic stress-indu-
ced oxidative damage. Horticulturae, 11: 477. https://
doi.org/10.3390/horticulturae11050477
Sabeem, M., Abdul Aziz, M., Mullath, S.K., Brini, F.,
Rouached, H., Masmoudi, K., 2022. Enhancing growth
and salinity stress tolerance of date palm using Pirifor-
mospora indica. Frontiers in Plant Science, 13: 1037273.
https://doi.org/10.3389/fpls.2022.1037273
Shareef, H.J., 2025. Date palm nutrition using trunk injection:
a comprehensive review. Asian Journal of Soil Science
and Plant Nutrition, 11 (1): 8–17. https://doi.org/10.9734/
ajsspn/2025/v11i1456
Shareef, H.J., Alhamd, A.S., Naqvi, S.A., Eissa, M.A., 2021a.
Adapting date palm offshoots to long-term irrigation
using groundwater in sandy soil. Folia Oecologica, 48
(1): 55–62. https://doi.org/10.2478/foecol-2021-0007
Shareef, H.J., Al-Tememi, I.H., Abdi, G., 2021b. Foliar nu-
trition of date palm : advances and applications. A review.
Folia Oecologica, 48 (1): 82–100. https://doi.org/10.2478/
foecol-2021-0010
Song, X., Chen, J., Xu, C., Cai, X., 2025. Physiological
and molecular mechanisms of exogenous salicylic acid
in enhancing salt tolerance in tobacco seedlings by regu-
lating antioxidant defence system and gene expression.
Frontiers in Plant Science, 16: 1545865. https://doi.org/
10.3389/fpls.2025.1545865
Swaid, S.Y., Shareef, H.J., 2025. Mitigating the oxidative
stress caused by UV-B in date palm seedlings: efficacy
of salicylic acid and ascorbic acid application. Folia
Oecologica, 52 (1): 34–41. https://doi.org/10.2478/foecol-
2025-0004
Tabatabai, M.A., 1998. Handbook of reference methods for
plant analysis. Crop Science, 38 (6): 1711. https://doi.org/
10.2135/cropsci1998.0011183x003800060050x
Väisänen, E., Takahashi, J., Obudulu, O., Bygdell, J.,
Karhunen, P., Blokhina, O., Laitinen, T., Teeri, T.H.,
Wingsle, G., Fagerstedt, K.V., Kärkönen, A., 2020.
Hunting monolignol transporters: membrane proteo-
mics and biochemical transport assays with membrane
vesicles of Norway spruce. Journal of Experimental
Botany, 71 (20): 6379–6395. https://doi.org/10.1093/
jxb/eraa368
Yuan, H., Shen, Q., Zheng, Y., Duan, M., Guo, J., Li, Y.,
300
Qiao, J., Huang, L., Tahira, M., Yin, Y., Hu, J., Zuo,
J., Yan, D., Zheng, B., Rao, M.J., 2026. Salicylic acid
mitigates drought-stress- induced oxidative damage in
Ilex rotunda through tissue-dspecific reprogramming of
antioxidant phenolics and ros scavenging. Antioxidants,
15: 808. https://doi.org/10.3390/antiox15070808
Zheng, M., Liu, Y., Zhang, G., Yang, Z., Xu, W., Chen, Q.,
2023. The applications and mechanisms of superoxide
dismutase in medicine, food, and cosmetics. Antioxi-
dants, 12 (9): 1675. https://doi.org/10.3390/antiox12091675
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