In vivo Volumetric, DTI and 1H MRS Rat Brain Protocol for Monitoring Early Neurodegeneration and Efficacy of the Used Therapy

Authors

  • Tomáš Tvrdík Central Laboratories, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia; Department of Radiology, Faculty of Medicine of Comenius University in Bratislava, Slovak Medical University and University Hospital Bratislava, Mickiewiczova 13, 81369 Bratislava, Slovakia https://orcid.org/0000-0003-0949-5348
  • Ľubomír Melicherčík Central Laboratories, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia; Department of Radiology, Faculty of Medicine of Comenius University in Bratislava, Slovak Medical University and University Hospital Bratislava, Mickiewiczova 13, 81369 Bratislava, Slovakia https://orcid.org/0000-0003-0642-9484
  • Katarína Šebeková Institute of Molecular Biomedicine, Faculty of Medicine of Comenius University in Bratislava, Sasinkova 4, 81108 Bratislava, Slovakia https://orcid.org/0000-0002-9641-9265
  • Jakub Szabó Institute of Molecular Biomedicine, Faculty of Medicine of Comenius University in Bratislava, Sasinkova 4, 81108 Bratislava, Slovakia https://orcid.org/0000-0002-6085-1291
  • Marianna Maková Central Laboratories, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia
  • Daniel Gogola Institute of Measurement Science, Slovak Academy of Sciences, Dúbravská cesta 9, 841 04 Bratislava, Slovakia
  • Svatava Kašparová Central Laboratories, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia

DOI:

https://doi.org/10.2478/msr-2023-0030

Keywords:

animals, multimodal imaging, in-vivo, diffusion tensor imaging (DTI), magnetic resonance spectroscopy (MRS)

Abstract

The aim of our study was to develop a multimodal experimental protocol for in vivo imaging and metabolic parameters (MRI, DTI and 1H MRS) in an animal model of neurodegeneration. We have successfully developed the protocol for simultaneous DTI/MRI/1H MRS measurement to ensure unaltered conditions for repeatable non-invasive experiments. In this experiment, diffusion tensor imaging, spectroscopic and volumetric “bio-markers” were generated in the brain for the D-galactose model of "age-related dementia". The hippocampal relative volume, taurine and myo-inositol relative concentrations were found to be significant predictors contributing to the differences between the groups of rats treated with D-galactose in simulated "neurodegeneration", even in response to the applied Huperzine A therapy.

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Published

17.10.2023

How to Cite

Tvrdík, T., Melicherčík, Ľubomír, Šebeková, K., Szabó, J., Maková, M., Gogola, D., & Kašparová, S. (2023). In vivo Volumetric, DTI and 1H MRS Rat Brain Protocol for Monitoring Early Neurodegeneration and Efficacy of the Used Therapy. Measurement Science Review, 23(5), 237–247. https://doi.org/10.2478/msr-2023-0030

Issue

Vol. 23 No. 5 (2023): Measurement Science Review

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Articles

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Copyright (c) 2023 Slovak Academy of Sciences - Institute of Measurement Science

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